The FC428 clone harbors a mosaic penA-allele, designated as PenA-60.001 by results of N. Modifications of penA, which encodes penicillin-binding protein 2 (PBP2), are a cause of cephalosporin resistance in N. We designed a real-time PCR to target unique sequences on the penA gene of the FC428 N. The study was approved by the University of Queensland Human Research Ethics Committee. Here, we describe a real-time PCR protocol to facilitate enhanced surveillance for the FC428 clone. Rapid and timely detection is pivotal to contain further spread of antimicrobial drug–resistant N.
However, there is now evidence of sustained international transmission of FC428, reported during 2017 in Canada ( 8) and Demark ( 9) (1 case each) and in Australia (2 cases) ( 10). gonorrhoeae had been rare and typically sporadic, including in 2009, H041 in Japan ( 2) in 2010, F89 in France ( 3) in 2011, F89 in Spain ( 4) in 2013, A8806 in Australia ( 5) in 2014, GU140106 in Japan ( 6) and in 2015, FC428 and FC460 in Japan ( 7). Until 2017, ceftriaxone-resistant strains of N. Therefore, the identification of any strains exhibiting resistance to ceftriaxone is of considerable public health concern.
Ceftriaxone, either monotherapy or in dual therapy with azithromycin, is the mainstay of treatment of patients diagnosed with Neisseria gonorrhoeae infection in most settings ( 1).
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